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How To Blue pippin: 6 Strategies That Work

Professional Freediver. Spouses. Audrey Mestre 1999-2002, Juscelina Cacau Melo 2012. Francisco Rodriguez, better known as Francisco 'Pipin' Ferreras (born January 18, 1962) is a Cuban free-diver known for his achievements in deep free diving and his relationship with his wife, Audrey Mestre .Pippin Hill Farm & Vineyards 2015 Easton Blue; Pippin Hill Farm & Vineyards 2019 Cabernet Sauvignon; Pippin Hill Farm & Vineyards 2019 Chardonnay Reserve; Pippin Hill Farm & Vineyards 2019 Wild Common Reserve; Pippin Hill Farm & Vineyards 2020 Chardonnay; Pippin Hill Farm & Vineyards 2020 Reserve Rosé; Pollak Vineyards 2019 Petit Verdot ...Pippin Dental is a medical group practice located in Elkhart, IN that specializes in Dentistry and Pediatric Dentistry. ... 0 Ratings. Insurance Check. Search for your insurance provider. Aetna; Ameritas; Anthem; Anthem Blue Cross Blue Shield; Assurant Health; BlueCross BlueShield of Tennessee; Cigna; Connection Dental; Delta Dental; Delta ...Blue Pippin Usage Form Date: Name: PI Name: Account #: Billing Contact: Billing Address: Cassettes Used (enter number): Any problems encountered during run? ITF Notes: Date Billed: Fill form: Try Risk Free. Form Popularity .为了对它们的生态学和进化有更多的了解,我们对三株从抑制病害土壤中分离出来的有很好抑菌效果的链霉菌菌株进行了全基因组测序,并将它们与之前测序的菌株进行比较。. 文章展示了从头测序(de novo sequencing)获得的3个链霉菌菌株的高质量全基因组 ...The size range of our cassettes can be found in our Pippin HT cassette kit reference chart. All PippinHT cassettes can accept up to 1.5 μg DNA per lane. All PippinHT cassettes can accept up to 1.5 μg DNA per lane. lected on a Sage Science Blue Pippin 1.5% cassette (Sage Science, Beverly, USA) to remove DNA molecules <600bp, and amplification-free, paired end libraries constructed usingPage 2 P/N 100-286-000-07 STEP Concentrate DNA Notes 1 Add 0.45X volume of AMPure® PB magnetic beads. _____ μL of sample X 0.45X = _____ μL of beads Note that the beads must be brought to room temperature and all AMPure PB bead Blue pippin size selection elution volume 07-03-2015, 02:20 AM. Hello, Has anyone has tried eluting their TruSeq DNA libraries in a smaller volume than the 40uL stated in the protocol? We carry out size selection on our TruSeq nano libraries after the PCR enrichment step which works for us but gives very low concentrations. I was wondering if ...This question is about the Blue Cash Everyday® Card from American Express @kendallmorris • 04/14/23 This answer was first published on 10/14/21 and it was last updated on 04/14/23.For the most current information about a financial product, ...The BluePippin is an updated version of Sage's Pippin Prep system, to which a pulsed-field power supply has been added. Pulsed field gel electrophoresis allows larger fragments of DNA separated with high resolution and accuracy. Using pre-cast gels, the BluePippin can automatically collect DNA targets up to 50 kilobase pairs, with minimal ...Sage Science一直没有停止创新的脚步,推出了PippinHT高通量型号,一次可处理多至24个样本,相当于5台Pippin PrepTM在同时运行。 特点: 和Pippin的其它型号一样,PippinHT也是基于预制胶技术,在一块预制胶上可以上样12个样本,通量更高且成本更低。We’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ... Automated microfluidic technologies have a great potential to increase the accessibility of COVID-19 diagnostic tests and to accelerate high-throughput detection processes, especially for POC testing. In addition, the automated microfluidic platforms developed for other pathogens ( 31 ), can be readily adapted to be used for COVID-19 diagnosis.The 20 kb templates used for sequencing were prepared by using Blue Pippin Size-Selection System (Sage Science, Beverly, MA) that allows resolving and collecting high molecular weight DNA, according to the manufacturer's instructions. DNA Sequencing and Assembly. The genomic sequence of Bt 2 Annotation of coding sequences (CDS), ribosomal RNA ...SDS-GHS: Reagent Replacement Kit for Dye Free Pippin Prep Cassettes (RIP2010, RIK2010, RIK 1510) SDS-GHS: Pippin Prep Cassettes with Ethidium Homodimer, Reagent Replacement Kit (REK2010) SDS-GHS: 10X KBB Electrophoresis Buffer (KBB1001) SDS-GHS 0.5X KBB buffer (PN190039) SDS-GHS: DNA Control Kits for Pippin Prep and BluePippin.Illumina paired-end library construction is the most popular use of the Pippin platform, and this is a typical citation. We are proud to have our products cited in the context of great research. Mate-pair sequencing: Optimization and cost-saving in tagmentation-based mate-pair library preparation and sequencingWe’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ... Then the optimized cycle number was used to generate double-stranded cDNA, followed by size selection using the Blue Pippin TM Size-Selection System to generate three libraries (1–2 kb, 2–3 kb ...BluePippin DNA size selection system. The Sage Science BluePippin system is an automated DNA size selection tool for NGS applications, plus the capability of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA up to 50 kb. For long-read sequencing applications, high-pass filtering allows users to collect all ...The Bioinformatics Core is offering in-depth analyses of PacBio sequence data. Samples can be provided as already isolated DNA by the customers. We do offer HMW-DNA isolation services for PacBio sequencing. Real-time data collection uses a 4 hour movie capture of the SMRT cell. Movies are processed in real time into trace files for each ZMW ...If you’re in the market for a used car, you may have heard of Blue Knob Auto Sales. This Pennsylvania-based dealership has been providing quality used cars for over 80 years. Before heading to Blue Knob Auto Sales, it’s important to do your...Flexible, movable furniture often feels temporary, missing the comfort factor – not so with Pippin. This single-seat, cone-shaped lounge chair offers a.Upgrading Pippin Instrument Software 1. Insert the USB drive with the upgrade folder into the USB port on the front panel of the BluePippin instrument. 2. From the Main Tab, press the "INFO" button. This will launch the information window. 3. From the Information Window, press "SOFTWARE UPGRADE", press"OK" at the warning prompt.Although they're called halflings, hobbits are a little more than half the height of your average human. They can range from two to four feet in height, but a typical hobbit is around three and a half feet tall. The notable exceptions to this rule are, of course, Merry and Pippin. MORE: Lord Of The Rings: 20 Most Powerful Beings, RankedGeneScan 500 ROX dye Size Standard, each. $2.25 (minimum fee $50) GeneScan 500 LIZ dye Size Standard, each. $2.25 (minimum fee $50) GeneScan 400HD ROX dye Size Standard, each. $2.25 (minimum fee $50) MapMarker X-Rhodamine Labeled 50-1000bp, each. $1.80 (minimum fee $50) GeneScan 1200 LIZ dye Size Standard, each.Page 1 PN 101-987-800 Version 02 (November 2021) Procedure & Checklist - Preparing HiFi SMRTbell® Libraries from Ultra-Low DNA Input . This procedure describes preparing HiFi SMRTbell libraries from 5 ng of input genomic DNA (gDNA) forDescription. The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long …Blue Pippin ™ Size Selection System (Sage Science, Beverly, MA) was used for the size selection of different sizes of cDNA libraries (1–2, 2–3, and > 3 kb). According to the instructions, three SMRT libraries were constructed using SMRTbell template prep kit 1.0 (Pacific Biosciences) and were sequenced through seven SMRT …Sheared genomic DNA was treated for damage repair and end repair, adaptor ligation, and size selection with a Blue Pippin system (Sage Science) to create SMRTbell template libraries.Description DNA Gel Cassettes Specifications Downloads Size Select Up To 24 Samples In One Run PippinHT incorporates the company’s highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The Blue Pippin has both standard and pulse field electrophoresis capability permitting a large range of fragment size selection. Cassettes can run up to 5 samples. Unused sample lanes can be used at a later time in some cases. Gel cassettes available are: 3% agarose 100-250bp isolation 2.5% agarose 100-600bp 1.5% agarose 250bp – 1.5KbFragment size may be measured using several methods (e.g., pulse-field, low percentage agarose gel analysis, blue pippin). This quality checkpoint . is important during the development and validation of the test. Labs may elect to omit this quality check after validation if the test has proven robust and stable.BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.Description DNA Gel Cassettes Specifications Downloads Size Select Up To 24 Samples In One Run PippinHT incorporates the company's highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin.The PippinHT system is the top choice for higher-throughput workflows. The PippinHT can process 11 size selections at once on a gel cassette (1 of the 12 lanes must be dedicated to an external size marker). The PippinHT accommodates 2 gel cassettes so up to 22 samples can be run at once, and at a quarter of the run time (1 hr 15 m) when ...22 thg 10, 2012 ... Our genomics core recently received a new Blue Pippin instrument. Another user and myself each prepared TruSeq DNA whole genome libraries ( ...Page 3 PN 100-377-300-03 2. Mix contents and spin the tubes briefly in a microcentrifuge. 3. Incubate the tubes at 72°C in a hot-lid thermal cycler for 3 min; slow ramp to 42°C at 0.1°C/sec let sit for 2最主要的是普通的切胶回收只能回收中小片段,不能回收三代平台所需的大片段。. 因此手工切胶回收正在被其他更安全简便的方法替代。. 其次是磁珠分选,磁珠分选在二代测序中应用比较广泛。. 原理是磁珠会优先抓取较大的核酸片段,在分选过程中,通过 ...Specifications. Concentration. 0.75%. Product Type. Agarose Gel Cassette. Quantity. 10 Pack. Provide Content Correction. Sage Science™ BluePippin™ Dye Free 0.75% Agarose Gel Cassettes are disposable and pre-cast cassettes with agarose and running buffer.Happy Birthday Card with Sprinkles in Blue. A fun card to send to a friend or family member and wish them a special day, perfect for kids and adults!Background Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes. No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop pests. In contrast, genomic information for crop pests is ...The authors report on a high quality genome assembly of the model plant species Arabidopsis include two telomere-to-telomere assemblies (Chr 3 and 5). PacBio's HiFi, Oxford Nanopore ultra-long reads and Hi-C (Illumina) were used. BluePippin was used to size select large fragments from genomic extracts and for Hi-FI library construction.Monarch HMW DNA Extraction Kit produces high molecular weight DNA with excellent yields, purity, and fragment length as compared to other commercially available kits. HMW was isolated from 1x106 HEK293 cells and fresh human blood with the kits indicated in the figure legend. Blood input volumes were used as specified in manufacturers ...For each Nanopore library, the gDNA was size-selected (10-50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION X5/PromethION sequencer ...For the Blue Pippin protocol, the SMRTbell templates were size-selected using the Sage Science BluePippin instrument with a 4 kb or 7 kb lower cutoff, depending on DNA quality. In both protocols, PacBio Sequencing Primer was then annealed to the SMRTbell template library, and sequencing polymerase was bound to them using Sequel Binding Kit 2.0. ...Blue chip shares are investments in well-established companies and leaders in their respective industries. Blue chip companies in general are financially stable with a history of solid earnings. Blue chip shares are investments in well-esta...Pipkin's Market, Montgomery, Ohio. 4,794 likes · 10 talking about this · 882 were here. Your neighborhood Farmer's Market, only better! Fresh Produce & Curated Garden CenterPippin Jelly Blue. Pippin Jelly Pink. Pippin Jelly Plain. Refrigerated Goods. ABC Bakery Supply Inc., offers a wide range of options of refrigerated goods for our customers in the bakery industry. We are known for our reliable, consistent and experienced distribution services, our expert product knowledge, and our professional dedication to ...A subset of the samples were sent to IBIS (n = 96, including 16 samples from each population) for PstI/MspI library preparation following the procedure of Poland et al. with the following modifications: addition of a size selection step using a Blue Pippin 2% agarose cassette with time settings to elute between 45 and 60 min and a plate barcode ...Individuals were barcoded with 48 unique barcodes (including negative controls with water as input), and we pooled and size-selected each of these subpools of 48 adapters with a Blue Pippin electrophoresis unit (Sage Science, Beverly, MA) using a 1.5% agarose gel cassette and set target size selection to the “tight 450 bp” setting.Sage Science develops products for the life sciences research markets. Our mission is to provide new systems to streamline and improve sample preparation workflows for DNA and protein analysis. In 2010-12, the company commercialized the Pippin Prep and BluePippin preparative electrophoresis systems for targeted size selection of biomolecules. The Pippin platform has since established itself as ...Solicitation Number: 75N95024Q00011 Title: DNA Size Selection (BluePippin Size Selection System) NAICS Code : 334516 Analytical Laboratory Instrument Manufacturing Description: This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations.Optionally, you can use Blue Pippin instead of the Agencourt AMPure XP purification beads to achieve a more precise size range in the epiGBS library, which would enable obtaining fewer loci for sequencing but with somewhat better coverage. Fig. 4.The DNA was sheared to approximately 20kb, size separated using a Blue Pippin using the PAC-30 KB cassette (Sage Science). Libraries were prepared for PacBio sequencing using the large insert library protocol and Illumina sequencing using the TruSeq PCR-free kit per manufacturer recommendations. Long read sequencing was conducted on the Pacific ...The Blue Pippin system . from Sage Science enables targeted size selection. High-pass filtering kits for the Blue Pippin are beneficial for long-read sequencing, as they allow the user to collect all the sample above a specified threshold, enabling analysis of only the fragment length of interest. Size selection therefore makes it possible toThe High Pass Plus™ gel cassette is the newest addition to the Pippin Family. As the name suggests, it is dedicated to our BluePippin "High-Pass" DNA size selection which has been a go-to method for increasing the read lengths for long-read sequencing. ... (100% up from standard Blue Pippin cassettes). We've also increased the size of ...A disabled black veteran who taught himself to paint, Pippin overcame his handicap — as well as discrimination — to become an overnight sensation in the American art world in the 1940s. Many consider him to be the foremost self-trained American artist of the 20th century, a painter whose artwork is reminiscent of the work of Grandma Moses ...It was then centrifuged at 5000 × g for 10 min at 4°. The solution was then purified, washed, and eluted using the Qiagen genome-tip kit instructions. Sequencing libraries were created by shearing DNA to 35 kb on a Megaruptor (Diagenode) and selecting for 18-50 kb using a Blue-Pippin (Blue Pippin system, Sage Science, Beverly, MA, USA).Molecular characterization is a key step in the risk assessment of genetically modified organisms (GMOs) for regulatory approval. Herein, we describe a method for analyzing copy number, insertion loci, and flanking sequences through whole-genome sequencing (WGS) and bioinformatics. Comprehensive molecular characterization of G2-6 transgenic rice was performed using this pipeline. The results ... Cassettes are disposable and pre-cast with agarose and running bBlue Pippin Productions first offering answers this que Using the Blue-Pippin Size-Selection System (Sage Science Inc., Beverly, MA, USA), we enriched and collected one fraction containing fragments >4 kb. The enriched fraction was mixed with unscreened fractions in an equimolar ratio to construct a mixed SMRT cell, which was subsequently run on the PacBio Sequel platform. ...Geographic variation in West African Agama picticauda: insights from genetics, morphology and ecology Sneha Krishnana, Caleb Ofori-Boatengb, Matthew K Fujitac and Adam D Leachéd aColorado State University, College of Veterinary Medicine and Biomedical Sciences 1350 Center Ave, Fort Collins, CO 80521, Fort Collins, CO, USA 80526, 4257858934; bKwame Nkrumah University of Science and The Sage Science portfolio includes products to perform DNA or cD Auntie Mabel is knitting a blue jumper for her sister Eadie when she runs out of wool. Frogs Pippin the dog meets some frogs in her garden, and Auntie Mabel finds some frogspawn.PippinHT Quick Guide HTC2010 Marker 20B. 1MB. PippinHT Quick Guide HTC1510 Marker 15C. 1MB. PippinHT Quick Guide HPE7510 Marker 75E 6-10Kb and 15-20kb High Pass. 948KB. PippinHT Quick Guide HPF7510 Marker 75F >30-40kb High Pass. 1MB. PippinHT Quick Guide HRT7510 9-30kb Range +T Marker 75E. Combine 30 μl of cleaned PCR product with 10 μl of ...

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A disabled black veteran who taught himself to paint, Pippin overcame his handicap — as well as discrimination — t...

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Pippin Gel Cassettes. Gel Cassettes are pre-cast with agarose, and use ethidium bromide to visualize DNA and provide more...

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Amplicons between 450 and 1500 bp in length were selected from purified pools using the Blue Pippin (Sage Science, United...

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Sep 19, 2018 · The High Pass Plus cassette has a stocky separation column, so DNA has a shorter di...

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